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KMID : 1134819980270020252
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Journal of the Korean Society of Food Science and Nutrition
1998 Volume.27 No. 2 p.252 ~ p.258
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Optimization of Culture Condition of Nocardia sp. L - 417 Strain for Biosurfactant Production
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Kim Soon-Han
Lim Ee-Jong
Lee Tae-Ho
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Abstract
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The strain producing biosurfactant was isolated from soil samples. The isolated strain was identified as the genus Nocardia through its morphological, cultural and physiological characteristics. A high concentration of the biosurfactant by Nocardia sp. L-417 was obtained after 4 days of cultivation in the culture medium containing 3% n-hexadecane, 0.1% NaNO₃, 0.02% K₂HPO₄, 0.01% H₂PO₄, 0.01% MgSO₄ㆍ7H₂O, 0.01% CaCl₂, 0.02% yeast extract, and 0.02% tryptone. The optimum pH and temperature for biosurfactant production were pH 6.0 and 30℃, respectively. Furthermore, most biosurfactants were produced during the exponential growth phase, and this fact indicated that the biosurfactants production was growth-associated. The biosurfactant showed the good emulsification activities on various emulsifying substrates such as bunker A, paraffin, corn oil and olive oil which are used widely in industries.
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KEYWORD
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biosurfactant, microbial emulsifier, emulsifying activity
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